A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination.

نویسندگان

  • Hideki Niimi
  • Masashi Mori
  • Homare Tabata
  • Hiroshi Minami
  • Tomohiro Ueno
  • Shirou Hayashi
  • Isao Kitajima
چکیده

To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 49 9  شماره 

صفحات  -

تاریخ انتشار 2011